期刊
BIORESOURCE TECHNOLOGY
卷 92, 期 2, 页码 201-208出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2003.06.002
关键词
phenolic compounds; wine industry by-product; liquid extraction; antioxidant activity; supercritical CO2
The recovery process of the phenolic compounds contained in the wine industry by-products and their antioxidant activity were examined in this work. To this purpose, a combined process of liquid and supercritical solvent extraction was employed. At first the effect of various process parameters of the liquid solvent extraction-the type of solvent and the pretreatment of the raw material (composition (skins, seeds, stems) and crushing)-on the antioxidant activity of the extract was examined. It was shown that an extract of a higher antioxidant activity was obtained by using ethyl acetate as solvent and raw material free of stems. These extracts were further treated with supercritical carbon dioxide (SC CO2) at various extraction pressures, which resulted in their significant enrichment in phenolic compounds and the improvement of their antioxidant and organoleptic properties, especially at pressures higher than 100 bar. The antioxidant activity of the products was determined by using the Rancimat method, as well as a simple and not time-consuming free radical method. It was proved that both the ethyl acetate extracts and those treated with SC CO2 had antioxidant activity comparable to that of antioxidants commonly used in industry, that is butylhydroxytoluene (BHT), a synthetic antioxidant, and Rosemary extract, a widely known natural one. (C) 2003 Elsevier Ltd. All rights reserved.
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