期刊
BLOOD
卷 103, 期 7, 页码 2522-2529出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2003-07-2439
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- NCI NIH HHS [P01CA71907-07, U01 CA84221-03, CA21765] Funding Source: Medline
The Runx1/core binding factor-beta (CBFbeta) transcriptional complex is required for the establishment of hematopoiesis during development. Despite its critical role during development, a detailed analysis of Runx1 expression within specific lineages and developmental stages of the adult hematopoietic system is lacking. To address this, we have developed a Runx1-green fluorescent protein (GFP) knock-in mouse. We show that Runx1 is expressed in several hematopoietic lineages, including myeloid, B-lymphoid, and T-lymphoid cells. By contrast, Runx1 is weakly expressed in early erythroid cells, and its expression is rapidly extinguished during later stages of erythropoiesis. Runx1 expression is induced during early B-cell development and is expressed at a uniform level during all subsequent stages of B-cell development. Within the thymus, Runx1 is expressed at the highest level in CD4(-)CD8(-) double-negative thymocytes. In peripheral T cells, Runx1 is differentially expressed, with CD4(+) T cells expressing 2- to 3-fold higher levels of Runx1 than CD8(+) cells. Taken together, these findings indicate that although widely expressed in the hematopoietic system, the expression of Runx1 is regulated in a cell type- and maturation stage-specific manner. In addition, the Runx1-IRES-GFP knock-in mouse strain should prove valuable for investigation of Runx1 function in adult hematopoiesis. (C) 2004 by The American Society of Hematology.
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