期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 34, 期 3, 页码 147-153出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2003.11.005
关键词
ganglioside G(M1); phospholipids; ternary system; atomic force microscopy; surface pressure; morphology
The membrane properties of the ganglioside G(M1) (GM1)/dioleoylphosphatidylcholine (DOPC) binary system and GM1/dipalmitoylphosphatidylcholine (DPPC)/DOPC ternary system were investigated using surface pressure measurements and atomic force microscopy (AFM), and the effect of surface pressure on the properties of the membranes was examined. Mixed GM1/DPPC/DOPC monolayers were deposited on mica using the Langmuir-Blodgett technique for AFM. GM1 and DOPC were immiscible and phase-separated. The AFM image of the GM1/DOPC (1:1) monolayer showed island-like GM1 domains embedded in the DOPC matrix. There was no morphological change on varying surface pressure. The surface pressure-area isotherm of the GM1/DPPC/DOPC (2:9:9) monolayer showed a two-step collapse as in the DPPC/DOPC (1:1) monolayer. The AFM image for the GM1/DPPC/DOPC monolayer showed DPPC and GM1 domains in the DOPC matrix, and the DPPC-rich phase containing GM1 showed a percolation pattern the same as the GM1/DPPC (1:9) monolayer. The percolation pattern in the GM1/DPPC/DOPC monolayer changed as the surface pressure was varied. The surface pressure-responsive change in morphology of GM1 was affected by the surrounding environment, suggesting that the GM1 localized in each organ has a specific role. (C) 2003 Elsevier B.V. All rights reserved.
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