期刊
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
卷 55, 期 1, 页码 187-197出版社
WILEY
DOI: 10.1002/prot.20005
关键词
new antigen receptor; bacteriophage display; shark antibody; Apical Membrane Antigen-1; malaria
The new antigen receptor (IgNAR) is an antibody unique to sharks and consists of a disulphide-bonded dimer of two protein chains, each containing a single variable and five constant domains. The individual variable (V-NAR) domains bind antigen independently, and are candidates for the smallest antibody-based immune recognition units. We have previously produced a library of V-NAR, domains with extensive variability in the CDR1 and CDR3 loops displayed on the surface of bacteriophage. Now, to test the efficacy of this library, and further explore the dynamics of V-NAR antigen binding we have performed selection experiments against an infectious disease target, the malarial Apical Membrane Antigen-1 (AMA1) from Plasmodium falciparum. Two related V-NAR, clones were selected, characterized by long (16- and 18-residue) CDR3 loops. These recombinant V(NAR)s could be harvested at yields approaching 5mg/L of monomeric protein from the E. coli periplasm, and bound AMA1 with nanomolar affinities (K-D = -2 x 10(-7) M). One clone, designated 12Y-2, was affinity-matured by error prone PCR, resulting in several variants with mutations mapping to the CDR1 and CDR3 loops. The best of these variants showed similar to10-fold enhanced affinity over 12Y-2 and was Plasmodium falciparum strain-specific. Importantly, we demonstrated that this monovalent V-NAR co-localized with rabbit anti-AMA1. antisera on the surface of malarial parasites and thus may have utility in diagnostic applications. (C) 2004 Wiley-Liss, Inc.
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