Deforestation alters the resource base and biomass of endemic stream insects in eastern Madagascar

1. Rainforest streams in eastern Madagascar have species-rich and diverse endemic insect communities, while streams in deforested areas have relatively depauperate assemblages dominated by collector-gatherer taxa. We sampled a suite of benthic insects and their food resources in three primary rainforest streams within Ranomafana National Park in eastern Madagascar and three agriculture streams in the park's deforested peripheral zone. We analysed gut contents and combined biomass and stable isotope data to examine stream community responses to deforestation in the region, which is a threatened and globally important hotspot for freshwater biodiversity. 2. Gut analyses showed that most taxa depended largely on amorphous detritus, obtained either from biofilms (collector-gatherers) or from seston (microfilterers). Despite different resource availability in forest versus agriculture streams, diets of each taxon did not differ between stream types, suggesting inflexible feeding modes. Carbon sources for forest stream insects were difficult to discern using delta(13)C. However, in agriculture streams dependence on terrestrial carbon sources was low relative to algal sources. Most insect taxa with delta(13)C similar to terrestrial carbon sources (e.g. the stonefly Madenemura, the caddisfly Chimarra sp. and Simulium blackflies) were absent or present at lower biomass in agriculture streams relative to forest streams. Conversely, collector-gatherers (Afroptilum mayflies) relied on algal carbon sources and had much higher biomass in agriculture streams. 3. Our analyses indicate that a few collector-gatherer species (mostly Ephemeroptera) can take advantage of increased primary production in biofilms and consequently dominate biomass in streams affected by deforestation. In contrast, many forest stream insects (especially those in the orders Plecoptera, Trichoptera and Diptera) depend on terrestrial carbon sources (i.e. seston and leaf litter), are unable to track resource availability and consequently decline in streams draining deforested landscapes. These forest-specialists are often micro-endemic and particularly vulnerable to deforestation. 4. The use of consumer biomass data in stable isotope research can help detect population-level responses to shifts in basal resources caused by anthropogenic change. We also suggest that restoration of vegetated riparian zones in eastern Madagascar and elsewhere could mitigate the deleterious effects of deforestation on sensitive, endemic stream taxa that are dependent on terrestrial carbon sources.