期刊
DEVELOPMENTAL BIOLOGY
卷 268, 期 1, 页码 135-151出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2003.12.010
关键词
blastocyst implantation; trophoblast; integrins; fibronectin; adhesion; migration
资金
- NCI NIH HHS [P30CA22453] Funding Source: Medline
- NIAAA NIH HHS [AA12057] Funding Source: Medline
- NICHD NIH HHS [HD 36764, HD37394, HD42636] Funding Source: Medline
- NIEHS NIH HHS [P30ES06639] Funding Source: Medline
During blastocyst implantation, interaction between integrins on the apical surface of the trophoblast and extracellular matrix (ECM) in the endometrium anchors the embryo to the uterine wall. Strong adhesion of the blastocyst to fibronectin (FN) requires integrin signaling initiated by exogenous fibronectin. However, it is not known how integrin signaling enhances blastocyst adhesion. We present new evidence that the integrin. alphaIIbbeta3, plays a key role in trophoblast adhesion to fibronectin during mouse peri-implantation development. Trafficking of alphaIIb to the apical surface of the trophoblast increased dramatically after blastocysts were exposed to fibronectin, whereas other fibronectin-binding integrins, alpha5beta1 and alphaVbeta3, were resident at the apical surface before ligand exposure. Functional comparisons among the three integrins revealed that ligation of alpha5beta1 most efficiently strengthened blastocyst fibronectin-binding activity, while subsequent trophoblast cell migration was dependent primarily on the beta3-class integrins. In vivo, alphaIIb was highly expressed by invasive trophoblast cells in the ectoplacental cone and trophoblast giant cells of the parietal yolk sac. These data demonstrate that trafficking of alphaIIb regulates adhesion between trophoblast cells and fibronectin as invasion of the endometrium commences. (C) 2004 Elsevier Inc. All rights reserved.
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