期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 48, 期 4, 页码 1242-1248出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.48.4.1242-1248.2004
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The anti-Candida activity of the innate defense protein human lactoferrin was investigated. Lactoferrin displayed a clear fungicidal effect against Candida albicans only under low-strength conditions. This candidacidal activity was inversely correlated with the extracellular concentration of the monovalent cations and was prevented by Na+ and K+ (greater than or equal to30 mM) and by divalent cations (Ca2+ and Mg2+ at greater than or equal to4 mM). A slight cellular release of K+, cytosolic acidification, and a change in the membrane potential were observed in C. albicans cells treated with lactoferrin, suggesting that this protein directly or indirectly interacts with the cytoplasmic membrane. Mitochondrial inhibitors (carbonyl cyanide m-chlorophenylhydrazone, 2,4-dinitrophenol, azide, and antimycin) as well as anaerobic conditions significantly reduced the killing effect of lactoferrin. These results suggest that low-strength conditions and the cellular metabolic state may modulate the candidacidal activity of human lactoferrin.
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