Two new arylnaphthalide lignans and antiplatelet constituents from Justicia procumbens

Two new arylnaphthalide lignans, procumphthalide A (1) and 4-O-beta-D-glucopyranosyl-(1'-->2)-beta-D-apiofuranosyldiphyllin, named procumbenoside B (2), along with cilinaphthalide B (3) and several other known compounds were isolated from the methanolic extracts of Justicia procumbens. By using NMR and other spectral methods, the structures of 1 and 2 were elucidated. Cilinaphthalide B (3), justicidin A (4), and taiwan E methyl ether (5) were shown to have an antiplatelet effect in human plateletrich plasma. In human citrated PRP, 5 showed a strong inhibitory effect on platelet aggregation induced by adrenaline in a concentration-dependent manner, with an IC50 value of about 27.6 muM.