4.5 Article

RAPD and SCAR markers linked to sex determination in Eucommia ulmoides Oliv.

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EUPHYTICA
卷 136, 期 3, 页码 233-238

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SPRINGER
DOI: 10.1023/B:EUPH.0000032741.99885.c4

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Eucommia ulmoides; RAPD; SCAR; sex determination

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Eucommia ulmoides Oliv. is strictly a dioecious perennial tree native to China. The pistillate plants are economically more useful than the staminate plants. The random amplified polymorphic DNA (RAPD) technique was used to screen markers of sex determination in this species. A 569 bp RAPD marker, marker linked to sex determination in E. ulmoides (MSDE), was found in all the pistillate but not in the staminate plants; its exclusiveness to pistillate plants was confirmed by Southern blotting. MSDE was sequenced and specific primers were synthesized to generate a 569 bp pistillate-specific SCAR marker, SCARmr. SCARmr could be useful for screening E. ulmoides plants for gender even before they reach reproductive maturity, resulting in considerable saving of time and economic resources.

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