期刊
ACTA PHYSIOLOGICA SCANDINAVICA
卷 180, 期 4, 页码 395-403出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1365-201X.2004.01259.x
关键词
Akt; protein kinase B; cell culture; ERK mitogen-activated protein kinase
类别
Aim: We hypothesized that myogenic differentiation of HSMC would yield a more insulin responsive phenotype. Methods: We assessed expression of several proteins involved in insulin action or myogenesis during differentiation of primary human skeletal muscle cultures (HSMC). Results: Differentiation increased creatine kinase activity and expression of desmin and myocyte enhancer factor (MEF)2C. No change in expression was observed for big mitogen-activated protein kinase (BMK1/ERK5), MEF2A, insulin receptor (IR), hexokinase II, and IR substrates 1 and 2, while expression of glycogen synthase, extracellular signal-regulated kinase 1 and 2 (ERK1/2 MAP kinase) and the insulin responsive aminopeptidase increased after differentiation. In contrast to protein kinase B (PKB)a, expression of (PKB)b increased, with differentiation. Both basal and insulin-stimulated PI 3-kinase activity increased with differentiation. Insulin-mediated phosphorylation of PKB and ERK1/2 MAP kinase increased after differentiation. Conclusion: Components of the insulin-signalling machinery are expressed in myoblast and myotube HSMC; however, insulin responsiveness to PKB and ERK MAP kinase phosphorylation increases with differentiation.
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