期刊
FEBS LETTERS
卷 563, 期 1-3, 页码 66-68出版社
WILEY
DOI: 10.1016/S0014-5793(04)00249-2
关键词
genetic regulation; lambda phage; non-specific binding; thermodynamics
We propose a thermodynamic model that includes the non-specific binding of the lambda. phage regulatory proteins CI and Cro. By fitting the model to experimental in vivo data on activities of the two promoters P-RM and P-R versus concentration, we estimate the free energy upon non-specific binding to be -4.1 +/- 0.9 kcal/mol for CI and -4.2 +/- 0.8 kcal/mol for Cro. For concentrations > 100 nM of CI or Cro, we find that > 50% of these proteins are non-specifically bound. In particular, in a lysogen (similar to250 CI monomeric equivalents per cell) nearly 90% of CI is non-specifically bound. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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