期刊
ANALYTICAL CHEMISTRY
卷 76, 期 8, 页码 2406-2410出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0354600
关键词
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资金
- NIGMS NIH HHS [R01 GM60562, R01 GM060562] Funding Source: Medline
A new generation of optically encoded beads has been prepared by using mesoporous polystyrene beads and surfactant-coated semiconductor quantum dots. In comparison with nonporous beads of similar sizes and chemical compositions, the encoded porous beads are similar to 1000 times brighter and 5 times more uniform in fluorescence intensities. Using both absolute intensity and ratiometric fluorescence coding, we show that the beads can be identified with a standard flow cytometer at 1000 beads/s. This result indicates that the multiple excited-state lifetimes and relaxation pathways of quantum dots do not limit their applications in high-speed optical detection and imaging.
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