期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1033, 期 2, 页码 267-273出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.01.053
关键词
stationary phases; LC; monoliths; polar hydroxylated aromatic compounds; amino acids
Porous monoliths based on N.N-dimethylacrylamide (DMAA) or methacrylamide (MAA) were prepared inside fused silica capillaries as stationary phases for nano-chromatography. The columns were characterized in terms of flow rate and backpressure and showed, e.g. differences as a function of the salt concentration added to the polymerization mixture. When the columns were investigated for the separation of uncharged (polar hydroxylated aromatic compounds) and charged (amino acids) analytes under pressure driven conditions (pLC), differences to the previously observed behavior under voltage driven conditions (CEC) were observed. Whereas the non-charged analytes showed similar behavior in both cases-thus, corroborating the previous assumption of a mainly chromatographic separation mode driven by hydrophilic interactions in CEC-the charged amino acids did not. Assuming that the separation was governed by chromatographic phenomena in the pLC mode and by both chromatographic and electrophoretic effects in the CEC mode, the experiments allowed deconvoluting the two contributions. In particular. the charged amino acids appeared to interact with the stationary phases mainly by electrostatic interactions modified by some hydrophilic effects. (C) 2004 Elsevier B.V. All rights reserved.
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