期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1033, 期 2, 页码 257-266出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.01.063
关键词
electrospray ionization; band broadening; principal component analysis; instrumentation; peptides
Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 mum i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min. (C) 2004 Elsevier B.V. All rights reserved.
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