期刊
AIDS
卷 18, 期 7, 页码 1067-1069出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00002030-200404300-00016
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Immortalized and transduced cell lines are traditionally used in models of the HIV-1 life cycle. Primary cells may better represent the tissue of origin and events in vivo. We utilized an HIV-1/murine leukemia A4070 pseudotype virus and overexpression of the transcriptional co-factor human Cyclin T1 to replicate HIV-1 in primary murine cells, and demonstrate that primary murine cells support HIV-1 infection better than immortalized cells.
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