期刊
JOURNAL OF COMPUTATIONAL CHEMISTRY
卷 25, 期 6, 页码 865-871出版社
WILEY
DOI: 10.1002/jcc.20011
关键词
SPICKER; near-native folds; TASSER
资金
- NIGMS NIH HHS [GM-37408] Funding Source: Medline
We have developed SPICKER, a simple and efficient strategy to identify near-native folds by clustering protein structures generated during computer simulations. In general, the most populated clusters tend to be closer to the native conformation than the lowest energy structures. To assess the generality of the approach, we applied SPICKER to 1489 representative benchmark proteins less than or equal to200 residues that cover the PDB at the level of 35% sequence identity; each contains up to 280,000 structure decoys generated using the recently developed TASSER (Threading ASSembly Refinement) algorithm. The best of the top five identified folds has a root-mean-square deviation from native (RMSD) in the top 1.4% of all decoys. For 78% of the proteins, the difference in RMSD from native to the identified models and RMSD from native to the absolutely best individual decoy is below 1 Angstrom the majority belong to the targets with converged conformational distributions. Although native fold identification from divergent decoy structures remains a challenge, our overall results show significant improvement over our previous Clustering algorithms. (C) 2004 Wiley Periodicals, Inc.
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