期刊
MAGNETIC RESONANCE IN MEDICINE
卷 51, 期 5, 页码 938-944出版社
WILEY
DOI: 10.1002/mrm.20072
关键词
endothelial progenitor cells; magnetic resonance; imaging; cell labeling; Gd/Eu-complexes; fluorescence microscopy
A simple labeling procedure of stem/progenitor cells based on the use of Gd-HPDO3A and Eu-HPDO3A, respectively, is described. The Gd-chelate acts as T-1-agent for MRI visualization, whereas the corresponding Eu-chelate acts as reporter in fluorescence microscopy. Owing to their substantial chemical equivalence, the two chelates are equally internalized in EPCs (endothelial progenitor cells), thus allowing their visualization by both techniques. The lanthanide chelates are entrapped in endosomic vesicles and the labeled cells retain biological activity with preservation of viability and pro-angiogenesis capacity. Hyperintense spots in MR have been observed for Gd-labeled EPCs injected under mice kidney capsule or grafted on a subcutaneous Matrigel plug up to 14 days after transplantation. Magn Reson Med 51:938-944, 2004. (C) 2004 Wiley-Liss, Inc.
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