期刊
JOURNAL OF PROTEOME RESEARCH
卷 3, 期 3, 页码 417-425出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr034071j
关键词
cross-linking; proteasome; PICUP
资金
- NIGMS NIH HHS [GM 58075] Funding Source: Medline
Ru(II)(bpy(2))(3)Cl-2+(2), ammonium persulfate, and visible light irradiation has been shown to rapidly and efficiently cross-link several interacting proteins. However, this methodology has not yet been used to map the architecture of large multi-protein complexes. In this study, this chemistry is applied to the crystallographically characterized yeast proteasome. The data obtained demonstrate both the method's increased generality and fidelity in comparison to traditional bifunctional cross-linking reagents, while also highlighting the future need for developing better analytical techniques to separate cross-linked products.
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