期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 42, 期 5, 页码 2197-2202出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.42.5.2197-2202.2004
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Clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16S rRNA gene sequence analysis. Each isolate yielded a greater than or equal to1,400-bp sequence containing < 5 ambiguities which was compared with the GenBank 16S rRNA gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (less than or equal to10 cases reported in the literature) human pathogens. Eleven new species, Actinobaculum massiliae, Candidatus Actinobaculum timonae, Paenibacillus sanguinis, Candidatus Bacteroides massiliae, Chryseobacterium massiliae, Candidatus Chryseobacterium timonae, Paenibacillus massiliensis, Candidatus Peptostreptococcus massiliae, Candidatus Prevotella massiliensis, Rhodobacter massiliensis, and Candidatus Veillonella atypica were identified. Sixteen species were obtained from humans for the first time. Our results show the important role that 16S rRNA gene sequence-based bacterial identification currently plays in recognizing unusual and emerging bacterial diseases.
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