期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 98, 期 5, 页码 856-861出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2003.10.021
关键词
DNA repair; AlkB; alpha-ketoglutarate; non-heme iron; dioxygenase; mass spectrometry; protein modeling
资金
- NIGMS NIH HHS [GM 54065, GM 063584, R01 GM063584] Funding Source: Medline
Escherichia coli AlkB is a DNA/RNA repair enzyme containing a mononuclear Fe(II) site that couples the oxidative decomposition of alpha-ketoglutarate (alphaKG) to the hydroxylation of 1-methyladenine or 3-methylcytosine lesions in DNA or RNA, resulting in release of formaldehyde and restoration of the normal bases. In the presence of Fe(II), alphaKG, and oxygen, but the absence of methylated DNA, AlkB was found to catalyze an aberrant reaction that generates a blue chromophore. The color is proposed to derive from Fe(III) coordinated by a hydroxytryptophan at position 178 as revealed by mass spectrometric analysis. Protein structural modeling confirms that Trp 178 is reasonably positioned to react with the Fe(IV)-oxo intermediate proposed to form at the active site. (C) 2003 Elsevier Inc. All rights reserved.
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