期刊
BIOMATERIALS
卷 25, 期 12, 页码 2319-2329出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2003.09.011
关键词
macroporous scaffold; thermally induced phase separation (TIPS); PLLA; PEG-PLLA diblock copolymer; MC3T3-E1 cell
A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5 wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300 mum. A macroporous PLLA scaffold was used to study an MC3T3-El cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks. (C) 2003 Elsevier Ltd. All rights reserved.
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