期刊
AQUATIC TOXICOLOGY
卷 128, 期 -, 页码 101-112出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquatox.2012.11.017
关键词
Cytochrome P450; CYP; AHR; Mytilus edulis; Blue mussel; Pollution
资金
- NOAA Sea Grant [NA10OAR4170086]
- NIH grant [5R01-ES015912]
- CAPES Ph.D. Fellowship
- CNPq Ph.D. Sandwich Fellowship, Brazil
Various sequencing projects over the last several years have aided the discovery of previously uncharacterized invertebrate sequences, including new cytochrome P450 genes (CYPs). Here we present data on the identification and characterization of two CYP1-like and three CYP3-like genes from the bivalve mollusk Mytilus edulis, and assess their potential as biomarkers based on their responses to several known vertebrate aryl hydrocarbon receptor (AHR) agonists. Quantitative real-time PCR was used to measure CYP transcript levels in digestive gland, labial palps, adductor muscle, gill, foot, and different regions of the Mantle. Levels of both CYP1-like genes were highest in digestive gland, whereas labial palps had the highest expression levels of the three CYP3-like genes followed by digestive gland and outer margin of the mantle. Mussels were exposed by injection to the AHR agonists, beta-naphthoflavone (BNF; 25 mu g g(-1)), 3,3',4,4',5-polychlorinated biphenyl (PCB126; 2 mu g g(-1)), or 6-formylindolo[3,2-b]carbazole (FICZ; 0.1 mu g g(-1)), or to Aroclor 1254 (a mixture of PCBs; 50 mu g g(-1)) for 24 h, followed by CYP expression analysis. There was no statistically significant change in expression of either of the CYP1-like genes after exposure to the various AHR agonists. The CYP3-like-1 gene was significantly up-regulated by BNF in gill tissues and the CYP3-like-2 gene was up-regulated in digestive gland by PCB126 and in gill tissue by BNF. These results suggest that distinct mechanisms of CYP gene activation could be present in M. edulis, although the importance of the CYP1-like and CYP3-like genes for xenobiotic and endogenous lipids biotransformation requires additional investigation. (c) 2012 Elsevier B.V. All rights reserved.
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