期刊
AQUATIC TOXICOLOGY
卷 103, 期 3-4, 页码 170-178出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2011.02.016
关键词
Estrogen; Fish; Microarray; Vitellogenin; Endocrine disruption; Aromatase
资金
- US Environmental Protection Agency (USEPA)
- University of Minnesota-Duluth
Prochloraz is a fungicide known to cause endocrine disruption through effects on the hypothalamic-pituitary-gonadal (HPG) axis. To determine the short-term impacts of prochloraz on gene expression and steroid production, adult female fathead minnows (Pimephales promelas) were exposed to the chemical (0 or 300 mu g/L) for a time-course of 6, 12 and 24 h. Consistent with inhibition of cytochrome P450 17 alpha-hydroxylase/17,20-lyase (CYP17) and aromatase (CYP19), known molecular targets of prochloraz, plasma 17 beta-estradiol (E2) was reduced within 6h. Ex vivo E2 production was significantly reduced at all time-points, while ex vivo testosterone (T) production remained unchanged. Consistent with the decrease in E2 levels, plasma concentrations of the estrogen-responsive protein vitellogenin were significantly reduced at 24h. Genes coding for CYP19. CYP17, and steroidogenic acute regulatory protein were up-regulated in a compensatory manner in ovaries of the prochloraz-treated fish. In addition to targeted quantitative real-time polymerase chain reaction analyses, a 15k feature fathead minnow microarray was used to determine gene expression profiles in ovaries. From time-point to time-point, the microarray results showed a relatively rapid change in the differentially expressed gene (DEG) profiles associated with the chemical exposure. Functional analysis of the DEGs indicated changes in expression of genes associated with cofactor and coenzyme binding (GO:0048037 and 0050662), fatty acid binding (GO:0005504) and organelle organization and biogenesis (GO:0006996). Overall, the results from this study are consistent with compensation of the fish HPG axis to inhibition of steroidogenesis by prochloraz, and provide further insights into relatively rapid, system-wide, effects of a model chemical stressor on fish. (C) 2011 Elsevier B.V. All rights reserved.
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