期刊
AQUATIC TOXICOLOGY
卷 100, 期 3, 页码 263-270出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2010.07.024
关键词
AhR; ER; Fish; Hepatocytes; Aromatic hydrocarbons; Estrogens
资金
- FORMAS [216-2007-468]
- University of Gothenburg
- Adlerbertska Forskningsstiftelsen, Helge Ax:sson Jonsons stiftelse and Wilhelm och Martina Lundgren Vetenskapsfond
The aryl hydrocarbon receptor (AhR) and the estrogen receptor (ER) are ligand-activated transcription factors, both of which can be activated by environmental pollutants. The AhR regulates cytochrome P450 1A (CYP1A) expression and can be induced by aromatic hydrocarbons. The ER regulates vitellogenin (VI-G) expression and can be induced by estrogenic substances. Both receptor responses are established biomarkers used to assess the effects of pollutants in the aquatic environment. The receptors can also be affected in situations of mixed exposure. Cross-talk between these receptor pathways has been suggested, although there are conflicting data in the literature. We investigated cross-talk between ER-VTG and AhR-CYP1A signaling pathways in primary cultures of rainbow trout hepatocytes, using quantitative PCR (qPCR) for mRNA analyses and studies of CYP1A catalytic function and protein expression. The model agonists beta-naphthoflavone (BNF) and 17 alpha-ethinylestradiol (EE2) were used for AhR and ER activation, respectively. Combined exposure to BNF and EE2 reduced the EE2-mediated induction of VTG mRNA levels by about 40%, but had no effect on the BNF-mediated CYP1A mRNA levels, indicative of a one-way inhibiting AhR-ER cross-talk. However, basal levels of CYP1A mRNA were reduced 40% upon exposure to EE2 alone, implying different cross-talk mechanism between basal and induced CYP1A mRNA levels. The mammalian ER antagonist fulvestrant (ICI) is commonly described as an absolute ER antagonist. However, ICI failed to reverse the ER activation caused by EE2 in the present study. The CYP1A-mediated ethoxyresorufin-O-deethylase (EROD) activity was reduced by 80% in cells co-treated with BNF and EE2, compared to cells exposed to BNF alone. In vitro inhibiting studies suggests that this reduction was a result of inhibition of the CYP1A catalyst by EE2 since EE2 acted as a potent inhibitor (IC50: 4.6 mu M) of the EROD activity. In addition, ICI also acted as a potent inhibitor of the EROD enzyme (IC50: 0.6 mu M). Taken together, our data supports a one-way inhibiting AhR-ER cross-talk in rainbow trout hepatocytes exposed to a mixture of BNF and EE2. (C) 2010 Elsevier B.V. All rights reserved.
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