期刊
AMINO ACIDS
卷 47, 期 7, 页码 1309-1318出版社
SPRINGER WIEN
DOI: 10.1007/s00726-015-1966-5
关键词
alpha-Ketoglutarate; Piglets; Lipopolysaccharide; Liver injury
资金
- National Basic Research Program of China [2012CB126305]
- National Natural Science Foundation of China [31372319, 31402084]
- Hubei Provincial Key Project for Scientific and Technical Innovation [2014ABA022]
- Hubei Provincial Research and Development Program [2010BB023]
- Natural Science Foundation of Hubei Province [2013CFA097, 2013CFB325, 2012FFB04805, 2011CDA131]
- Scientific Research Program of Hubei Provincial Department of Education [D20141701]
- Hubei Hundred Talent Program
- Agriculture and Food Research Initiative Competitive Grants of USDA National Institute of Food and Agriculture [2014-67015-21770]
- Texas AgriLife Research [H-82000]
- NIFA [687803, 2014-67015-21770] Funding Source: Federal RePORTER
There is growing interest in glutamate as a functional amino acid in nutrition and health. This study was conducted to determine whether glutamate precursor alpha-ketoglutarate (AKG) could alleviate lipopolysaccharide (LPS)-induced liver injury in young pigs. Twenty-four piglets were randomly assigned to the control, LPS, or LPS + AKG group. Piglets in the control and LPS groups were fed a basal diet, whereas piglets in the NAC group were fed the basal diet supplemented with 1 % AKG. On days 10, 12, 14, and 16 of the trial, piglets in the LPS and LPS + AKG groups received intraperitoneal administration of LPS (80 mu g/kg BW), whereas piglets in the control group received the same volume of saline. On day 16 of the trial, blood samples were collected 3 h after LPS or saline injection. Twenty-four hours post-administration of LPS or saline (on day 17 of the trial), piglets were killed to obtain liver for analysis. Dietary AKG supplementation alleviated LPS-induced histomorphological abnormalities and mitigated LPS-induced increases in aspartate aminotransferase (AST) activity and AST/ALT ratio (P < 0.05). Compared with the LPS group, dietary supplementation with AKG decreased plasma glutamate concentration, while increasing hepatic concentrations of glutamate, glutamine, leucine, asparagine, lysine, alanine, serine, threonine, valine, and phenylalanine (P < 0.05). LPS challenge dramatically increased concentrations of malondialdehyde and decreased glutathione peroxidase activity in the liver. Additionally, LPS challenge enhanced concentrations of AMP and total protein, as well as RNA/DNA and total protein/DNA ratios, while decreasing hepatic ADP concentrations. These adverse effects of LPS challenge were ameliorated by AKG supplementation. Collectively, dietary AKG supplementation provides a new means to ameliorate LPS-induced liver injury by increasing anti-oxidative capacity and improving energy metabolism in young pigs.
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