期刊
AQUATIC TOXICOLOGY
卷 90, 期 3, 页码 223-227出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2008.09.002
关键词
Pollution; Biodegradation; Microcosms; T-RFLP; Polycyclic aromatic hydrocarbons
资金
- Stockholm marine research centre (SMF)
The ecological consequences of antibiotics in the aquatic environment have been an issue of concern over the past years due to the potential risk for negative effects on indigenous microorganisms. Microorganisms provide important ecosystem services, such as nutrient recycling, organic matter mineralization and degradation of pollutants. In this study, effects of exposure to the antibiotic ciprofloxacin on the bacterial diversity and pollutant degradation in natural marine sediments were studied using molecular methods (T-RFLP) in combination with radiorespirometry. In a microcosm experiment, sediment spiked with C-14-labelled pyrene was exposed to five concentrations of ciprofloxacin (0, 20, 200, 1000 and 2000 mu g L-1) in a single dose to the overlying water. The production of (CO2)-C-14 (i.e. complete mineralization Of pyrene) was measured during 11 weeks. Sediment samples for bacterial community structure analysis were taken after 7 weeks. Results showed a significant dose-dependent inhibition of pyrene mineralization measured as the total (CO2)-C-14 production. The nominal EC50 was calculated to 560 mu g L-1, corresponding to 0.4 mu g/kg d.w. sediment. The lowest effect concentration on the bacterial community structure was 200 mu g L-1, which corresponds to 0.1 mu g/kg d.w. sediment. Our results show that antibiotic pollution can be a potential threat to both bacterial diversity and an essential ecosystem service they perform in marine sediment. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据