期刊
AQUATIC TOXICOLOGY
卷 89, 期 3, 页码 158-166出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquatox.2008.06.011
关键词
Tigriopus japonicus; Glutathione S-transferase biomarker; Trace metal exposure; Antioxidant defense
资金
- Ministry of Land, Transportation and Maritime Affairs, Republic of Korea
- KOSEF [R0A-2006-000-10155]
- Eco-Technopia 21
- National Research Foundation of Korea [2006-01926, 과06A1507] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The intertidal copepod, Tigriopus japonicus has been recognized as a potential model species for marine pollution toxicity testing. Toxicity ranges of several biocides, endocrine-disrupting chemicals (EDCs), and trace metals are known in T. japonicus. A large number of expressed sequence tags (ESTs) and genomic DNA are also sequenced from T. japonicus. In this study, expression of ten glutathione S-transferase (GST) genes was studied in the copepods exposed to trace metals. Expression of these genes was also studied against exposure to hydrogen peroxide (H2O2) used as a positive control with prooxidant activity. Of all genes, expression of GST-Sigma (GSTS) was highly upregulated in H2O2 as well as trace metal-exposed copepods. In the time-course study, expression of GSTS mRNA was more consistent compared to other GSTs such as GST-Omega, GST-Delta1, GST-Theta3 or microsomal GST1 (mGST1). GSTS is predominantly reported from the insects. Coupled with the previous study of the in vitro antioxidant role of T. japonicus GSTS, these findings imply all antioxidant role for GSTS and highlight its importance as a biomarker of exposure to. Japonicus. However, further validation and field trials Would be necessary to propose trace metals in GSTS gene expression as biomarker of exposure to trace metals, as for some trace Metals such as silver the response was not consistent in concentration and time-series exposure experiments. (c) 2008 Elsevier B.V. All rights reserved.
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