期刊
DEVELOPMENTAL DYNAMICS
卷 230, 期 1, 页码 91-99出版社
WILEY-BLACKWELL
DOI: 10.1002/dvdy.20035
关键词
BrdU; cell cycle; H3; histone; hydroxyurea; macrostomum; Platyhelminthes; stem cells; turbellaria
Development, growth, and regeneration in Macrostomum are based-as in all Platyhelminthes- on likely totipotent stem cells (neoblasts), basic for all Bilaterians. We demonstrate dynamics and migration of neoblasts during postembryonic development, starvation, and feeding of Macrostomum sp. Double labeling of S-phase and mitotic cells revealed a fast cell turnover. Conflicting with recent results from planarians, we have some indication of slow cycling neoblasts. As in planarians, starvation dramatically reduced mitotic activity and a very basic level was maintained after 30 days of starvation. Afterward, feeding induced a dramatic immediate proliferative response probably caused by G2-arrested neoblasts. The following 12 hr showed a significant mitotic decline, caused by the depletion of the G2 neoblast pool. Neoblasts that pass through S-phase led to a maximum of mitoses after 48 hr. Our results allow deeper insight into cellular dynamics of an ancestral bilaterian stem cell system of a basal Platyhelminth. (C) 2004 Wiley-Liss, Inc.
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