FT-IR study of the effect of lead and the influence of chelating agents, DMSA and D-Penicillamine, on the biochemical contents of brain tissues of Catla catla fingerlings

The goal of this study was to investigate the effect of lead and the influence of chelating agents,meso 2, 3-dimercaptosuccinic acid (DMSA) and D-Penicillamine, on the biochemical contents of the brain tissues of Catla catla fingerlings by Fourier Transform Infrared Spectroscopy. FT-IR spectra revealed significant differences in absorbance intensities between control and lead-intoxicated brain tissues, reflecting a change in protein and lipid contents in the brain tissues due to lead toxicity. In addition, the administration of chelating agents, DMSA and D-Penicillamine, improved the protein and lipid contents in the brain tissues compared to lead-intoxicated tissues. Further, DMSA was more effective in reducing the body burden of lead. The protein secondary structure analysis revealed that lead intoxication causes an alteration in protein profile with a decrease in alpha-helix and an increase in beta-sheet structure of Catla catla brain. In conclusion, the study demonstrated that FT-IR spectroscopy could differentiate the normal and lead-intoxicated brain tissues due to intrinsic differences in intensity.