期刊
JOURNAL OF MASS SPECTROMETRY
卷 39, 期 5, 页码 505-513出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.611
关键词
Lipopolysaccharide; lipid A; Pseudomonas aeruginosa; electrospray ionization mass spectrometry; ion-trap analyzer
The lipid A components of the Pseudomonas aeruginosa strains PAO1 (wild-type) and derived mutants PAO1 algC::tet and PAO1 PDO100 were isolated after mild acetic acid hydrolysis of LPS. Their structural heterogeneities were characterized using electrospray ionization (ESI) ion-trap mass spectrometry (MS) with direct infusion in the negative ion mode without prior derivatization. The ESI-mass spectra revealed monophosphorylated molecules corresponding to known tetra-, penta- and hexaacylated structures of P. aeruginosa lipid A. The MS/MS fragmentation patterns allowed the location of fatty acyl chains on the disaccharide backbone of lipid A. In addition, a hexaacylated lipid A containing a hexadecanoyl chain was detected for the first time in strain P. aeruginosa PAO1. With multiple stages of fragmentation (MSn), the position of this hexadecanoyl chain O-linked to the decanoyl chain at the C-3' position of the glucosamine backbone was determined. This sensitive method is suitable to reveal lipid A heterogeneity, i.e. the nature, number and distribution of acyl chains, without prior lipopolysaccharide purification. The lipid A from mutant strains were also characterized and significant differences were shown in the abundance of monophosphorylated lipid A components between the wild-type and the mutant strains. Copyright (C) 2004 John Wiley Sons, Ltd.
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