Pseudomonas lutea sp nov., a novel phosphate-solubilizing bacterium isolated from the rhizosphere of grasses

A phosphate-solubilizing bacterial strain designated OK2(T) was isolated from rhizospheric soil of grasses growing spontaneously in a soil from Spain. Cells of the strain were Gram-negative, strictly aerobic, rod-shaped and motile. Phylogenetic analysis of the 16S rRNA gene indicated that this bacterium belongs to the gamma-subclass of Proteobacteria within the genus Pseudomonas and that the closest related species is Pseudomonas graminis. The strain produced catalase but not oxidase. Cellulose, casein, starch, gelatin and urea. were not hydrolysed. Aesculin was hydrolysed. Growth was observed with many carbohydrates as carbon sources. The main non-polar fatty acids detected were hexadecenoic acid (116: 1), hexadecanoic acid (116: 0) and octadecenoic acid (118: 1). The hydroxy fatty acids detected were 3-hydroxydecanoic acid (3-OH 10:0), 3-hydroxydodecanoic acid (3-OH 12:0) and 2-hydroxydodecanoic acid (2-OH 12:0). The G + C DNA content determined was 59.3 mol%. DNA-DNA hybridization showed 48.7% relatedness between strain OK2(T) and P. graminis DSM 11363(T) and 26.2% with respect to Pseudomonas rhizosphaerae LMG 21640(T). Therefore, these results indicate that strain OK2(T) (= LMG 21974(T) = CECT 5822(T)) belongs to a novel species of the genus Pseudomonas, and the name Pseudomonas lutea sp. nov. is proposed.