期刊
BIOPOLYMERS
卷 74, 期 1-2, 页码 168-171出版社
JOHN WILEY & SONS INC
DOI: 10.1002/bip.20065
关键词
infrared microspectroscopy; cultured cervical cancer cells; cell cycle
资金
- NCI NIH HHS [R01 CA090346-01A2, CA 090346, R01 CA090346] Funding Source: Medline
- NCRR NIH HHS [G12 RR003037, GRR-03037] Funding Source: Medline
- NIGMS NIH HHS [S06 GM060654, GM 60654] Funding Source: Medline
We report infrared (IR) spectra observed,for individual, cultured human cervical cancer (HeLa) cells. Spectra were collected microscopically, in reflection/absorption modes, from cells deposited and dried on microscope slides or from cells grown directly on slides. Within the spectra of the dried cells, significant spectral heterogeneity exists that was previously attributed to different stages of the cell cycle [Boydston-White. S., et al., Biospectroscopy 1999, 5, 219-227; Holman, H. Y., et al., Biopolymers Biospectrosc 2000, 57, 329-335; Tobin, M. J., et al., in First BASIE Workshop, Karlsruhe, Germany, 2003]. The results reported here confirm earlier findings and present the possibility of determining the abundance of cells within each stage of the cycle from the IR spectra. In an accompanying paper, we show that the spectra of cells in suspension exhibit spectral intensity distributions that are different from that of the dried cells. This result has far-reaching implications,for the use of infrared microspectroscopy to screen dried cell preparations for the presence of abnormal cells. (C) 2004 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据