Reinekea marinisedimentorum gen. nov., sp nov., a novel gammaproteobacterium from marine coastal sediments

A Gram-negative, oxidase- and catalase-positive, rod-shaped bacterium, designated strain KMM 3655(T), was isolated from a coastal marine sediment sample. The novel bacterium required sodium ions for growth and grew between 0.5 and 5% NaCl and at 4-37degreesC, but not at 40degreesC. It reduced nitrate, formed acids from glucose under aerobic and anaerobic conditions, utilized a limited spectrum of organic substrates and did not produce gelatinase, caseinase, amylase or chitinase. The major isoprenoid quinone was Q8. Polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown phospholipid. Fatty acid analysis of strain KMM 3655(T) revealed C-16:0, C(16:1)omega7c and C(18:1)omega7c as predominant components. The G+C content of the DNA was 51.1 mol%. Phylogenetic analysis of the 16S rDNA sequence placed the new isolate within the gamma-Proteobacteria as a separate deep branch, with about 90% sequence similarity to representatives of the genus Oceanospirillum and other remotely related genera. Combined phylogenetic and physiological data show that the new marine sediment isolate, KMM 3655(T), represents a novel genus and species, for which the name Reinekea marinisedimentorum gen. nov., sp. nov. is proposed. The type strain is KMM 3655(T) (=DSM 15388(T)).