期刊
JOURNAL OF MICROSCOPY-OXFORD
卷 214, 期 -, 页码 174-181出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.0022-2720.2004.01347.x
关键词
autofluorescence; confocal microscopy; multiphoton microscopy; spectral imaging; unmixing
类别
Fluorescence imaging at high spectral resolution is now a practical reality and has great promise in plant cell biology. Emission spectral curve data can be used computationally to distinguish spectrally similar fluorophores, or to remove autofluorescence, and to spectrally analyse autofluorescent molecules, which are especially abundant in plant tissues. Examples of these applications in plant cells are given, and a comparison is made between the current offerings in spectral imaging laser scanning confocal microscopes.
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