期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 76, 期 3, 页码 356-362出版社
WILEY
DOI: 10.1002/jnr.20077
关键词
exercise; hippocampus; circuitry; BDNF; synapsin I; immunohistochemistry
资金
- NINDS NIH HHS [NS 38978, NS 39522] Funding Source: Medline
To assess the relationship between brain-derived neurotrophic factor (BDNF) and synapsin I in the hippocampus during exercise, we employed a novel microsphere injection method to block the action of BDNF through its tyrosine kinase (Trk) receptor and subsequently measure the mRNA levels of synapsin I, using real-time TaqMan RT-PCR for RNA quantification. After establishing a causal link between BDNF and exercise-induced synapsin I mRNA levels, we studied the exercise-induced distribution of BDNF and synapsin I in the rodent hippocampus. Quantitative immunohistochemical analysis revealed increases of BDNF and synapsin I in CA3 stratum lucidum and dentate gyrus, and synapsin I alone in CA1 stratum radiatum and stratum laconosum moleculare. These results indicate that exercise induces plasticity of select hippocampal transsynaptic circuitry, possibly comprising a spatial restriction on synapsin I regulation by BDNF. (C) 2004 Wiley-Liss, Inc.
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