期刊
FEBS LETTERS
卷 565, 期 1-3, 页码 163-166出版社
WILEY
DOI: 10.1016/j.febslet.2004.03.096
关键词
actin; jasplakinolide; phalloidin; fluorescence resonance energy transfer; differential scanning calorimetry
In this work the effect of phalloidin and jasplakinolide on the dynamic properties and thermal stability of actin filaments was studied. Temperature dependent fluorescence resonance energy transfer measurements showed that filaments of Ca-actin became more rigid in the presence of phalloidin or jasplakinolide. Differential scanning calorimetric data implied that the stiffer filaments also had greater thermal stability in the presence of phalloidin or jasplakinolide. The fluorescence and calorimetric measurements provided evidences that the extent of stabilization by jasplakinolide was greater than that by phalloidin. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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