期刊
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
卷 309, 期 2, 页码 720-729出版社
AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
DOI: 10.1124/jpet.103.061077
关键词
-
资金
- NCRR NIH HHS [RR-00054] Funding Source: Medline
- NIA NIH HHS [AG-17880] Funding Source: Medline
- NIDA NIH HHS [DA-05258, DA-13209, DA-13834] Funding Source: Medline
- NIDDK NIH HHS [DK/AI-58496] Funding Source: Medline
- NIMH NIH HHS [MH-58435, MH-34223, MH-01237] Funding Source: Medline
We evaluated the effect of age on CYP3A expression and function in the liver, intestine, and kidney from young (3-4 months), intermediate (13-14 months), and old (25-26 months) male Fischer-344 rats. The biotransformation of triazolam to its primary hydroxylated products, 4-OH-TRZ (triazolam) and alpha-OH-TRZ, was used as a marker of CYP3A activity in rat liver and intestine. Immunoactive CYP3A expression was evaluated by Western blot analysis in the rat intestine, liver, and kidney. Since testosterone and NADPH reductase levels may modulate CYP3A activity, we also examined free plasma testosterone concentrations and NADPH reductase expression in these rats. The effect of age on CYP3A expression was tissue-specific.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据