期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 55, 期 400, 页码 1187-1193出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erh135
关键词
Arabidopsis; drought; EMS mutants; infrared thermography; low humidity
资金
- Biotechnology and Biological Sciences Research Council [P18229] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [P18229] Funding Source: Medline
The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.
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