期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 70, 期 5, 页码 3171-3175出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.5.3171-3175.2004
关键词
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Bifidobacteria are one of the most common bacterial types found in the intestines of humans and other animals and may be used as indicators of human fecal pollution. The presence of nine human-related Bifidobacterium species was analyzed in human and animal wastewater samples of different origins by using species-specific primers based on 16S rRNA sequences. Only B. adolescentis and B. dentium were found exclusively in human sewage. A multiplex PCR approach with strain-specific primers was developed. The method showed a sensitivity threshold of 10 cells/ml. This new molecular method could provide useful information for the characterization of fecal pollution sources.
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