期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 199, 期 9, 页码 1213-1221出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20031462
关键词
lymphocytes; tumor-infiltrating; T lymphocyte subsets; cell movement; immunologic surveillance
资金
- NCI NIH HHS [CA81403, P01 CA081403, CA22794] Funding Source: Medline
CD1d-restricted Valpha24-Jalpha18-invariant natural killer T cells (iNKTs) are potentially important in tumor immunity. However, little is known about their localization to tumors. We analyzed 98 untreated primary neuroblastomas from patients with metastatic disease (stage 4) for tumor-infiltrating iNKTs using TaqMan((R)) reverse transcription polymerase chain reaction and ofluorescent microscopy. 52 tumors (53%) contained iNKTs, and oligonucleotide microarray analysis of the iNKT(+) and iNKT(-) tumors revealed that the former expressed higher levels of CCL2/MCP-1, CXCL12/SDF-1, CCL5/RANTES, and CCL21/SLC. Eight tested neuro blastoma cell lines secreted a range of CCL2 (0-21.6 ng/ml), little CXCL12 (less than or equal to0.1 ng/ml), and no detectable CCL5 or CCL21. CCR2, the receptor for CCL2, was more frequently expressed by iNKT compared with natural killer and T cells from blood (P < 0.001). Supernatants of neuroblastoma cell lines that produced CCL2 induced in vitro migration of iNKTs from blood of patients and normal adults; this was abrogated by an anti-CCL2 monoclonal antibody. CCL2 expression by tumors was found to inversely correlate with MYCN proto-oncogene amplification and express on (r = 0.5, P < 0.001), and MYCN-high/CCL2-low expression accurately predicted. the absence of iNKTs (P < 0.001). In summary, iNKTs migrate toward neuroblastoma cells in a CCL2-dependent manner, preferentially infiltrating MYCN nonamplified tumors that express CCL2.
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