期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 86, 期 3, 页码 273-276出版社
WILEY
DOI: 10.1002/bit.20033
关键词
cross-linked enzyme aggregates (CLEAs); nitrilase; oxynitrilase; alcohol dehydrogenase; penicillin G acylase; dextran; cross-linker
Cross-linked enzyme aggregates (CLEAs) were prepared from several enzymes (penicillin G acylase, hydroxynitrile lyase, alcohol dehydrogenase, and two different nitrilases) by precipitation and subsequent cross-linking using dextran polyaldehyde. In most cases, higher immobilization yields were obtained using the latter cross-linker as compared with the commonly used glutaraldehyde. Active site titration of penicillin acylase CLEAs showed that the higher activity originated from a significantly lower loss in active sites using dextran polyaldehyde as a cross-linking agent. It is proposed that macromolecular cross-linkers are too large to penetrate the protein active site and react with catalytically essential amino acid residues. (C) 2004 Wiley Periodicals, Inc.
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