期刊
JOURNAL OF CELL BIOLOGY
卷 165, 期 3, 页码 313-321出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200311076
关键词
ER export site; fluorescence recovery after photobleaching; GRASP; organelle biogenesis; basal body
类别
资金
- Wellcome Trust Funding Source: Medline
Duplication of the single Golgi apparatus in the protozoan parasite Trypanosoma brucei has been followed by tagging a putative Golgi enzyme and a matrix protein with variants of GFP. Video microscopy shows that the new Golgi appears de novo, near to the old Golgi, about two hours into the cell cycle and grows over a two-hour period until it is the same size as the old Golgi. Duplication of the endoplasmic reticulum (ER) export site follows exactly the same time course. Photobleaching experiments show that the new Golgi is not the exclusive product of the new ER export site. Rather, it is supplied, at least in part, by material directly from the old Golgi. Pharmacological experiments show that the site of the new Golgi and ER export is determined by the location of the new basal body.
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