期刊
CANCER RESEARCH
卷 64, 期 10, 页码 3479-3485出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-3299-2
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资金
- NCI NIH HHS [P30-CA16672, P01-CA099031, 2R01-CA60488] Funding Source: Medline
The origin of cell lines is critical in defining cell type-specific biological functions. Several reports (D. T. Ross et al, Nat Genet 2000;24:227-35; G. Ellison et al., J Clin Pathol Mol Pathol 2002;55:294-9) suggested that the MDA-MB-435 cell line, a cell line extensively used for studying breast cancer biology, has a gene expression pattern most compatible with melanocyte origin. However, we demonstrate that MDA-MB-435 cells express breast-specific or epithelial-specific markers. Also, MDA-MB-435 cells were induced to express breast differentiation-specific proteins and secrete milk lipids as observed in other well-established breast cancer cell lines. Notably, MDA-MB-435 cells also expressed melanocyte-specific proteins as did another highly aggressive breast cancer cell line. MDA-MB-435 xenograft tissue sections stained entirely positive for epithelium-specific markers but only partially positive for melanocyte-specific markers. Thus, MDA-MB-435 is most likely a breast epithelial cell line that has undergone lineage infidelity.
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