期刊
VETERINARY MICROBIOLOGY
卷 100, 期 1-2, 页码 11-16出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1135(03)00126-3
关键词
Clostridium perfringens; toxin genes; enterotoxin; beta(2)-toxin; multiplex PCR
In this study we provide a protocol for genotyping Clostridium perfringens with a new multiplex PCR. This PCR enables reliable and specific detection of the toxin genes cpa, cpb, etx, iap, cpe and cpb2 from heat lysed bacterial suspensions. The efficiency of the protocol was demonstrated by typing C. perfringens reference strains and isolates from veterinary bacteriological routine diagnostic specimens. (C) 2003 Elsevier Science B.V. All rights reserved.
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