期刊
FEBS LETTERS
卷 566, 期 1-3, 页码 218-222出版社
WILEY-BLACKWELL
DOI: 10.1016/j.febslet.2004.04.035
关键词
copper; P-type ATPase; CPx-ATPase; P-IB-ATPase; CCC2; RAN1
Copper transport across membranes plays an important role in plant growth and survival. P-1B-type ATPases participate in transmembrane transport of copper in various organisms. A Brassica napus cDNA (BnRAN1) encoding a putative Cu2+-ATPase was cloned in this study. A complementation assay demonstrated that the protein encoded by this cDNA could functionally replace Ccc2p, a Saccharomyces cerevisiae Cu2+-ATPase, rescuing growth of ccc2 mutant under iron-limited conditions. Our results suggest that this rescue likely resulted from restoration of copper delivery, mediated by BnRAN1, to Fet3p. This study is amongst the first to demonstrate that a putative plant P-1B-ATPase is functional and to examine its substrate specificity. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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