期刊
FEBS LETTERS
卷 566, 期 1-3, 页码 25-29出版社
WILEY
DOI: 10.1016/j.febslet.2004.03.113
关键词
NMDA-receptor; filipin; methyl-beta-cyclodextrin; hippocampal cell; lipid raft
The present study was carried out to investigate the potential involvement of cholesterol-rich membrane microdomains in the mobilization of calcium induced by NMDA-receptors (NMDA-R). We herein provide evidence that agents interfering with plasma membrane cholesterol (namely, filipin and methyl-beta-cyclodextrin (Cdex)) inhibit the NMDA-stimulated influx of calcium in hippocampal cells in culture. Filipin-treated cells maintained their morphology and were able to respond with a calcium influx to high K+ challenge, whereas Cdex altered both cellular parameters. These results suggest that the NMDA-R can be located in cholesterol-rich membrane microdomains or alternatively that the mechanisms coupling their dynamics in the post-synaptic membrane are dependent on the integrity of the microdomains. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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