H3 mRNA level as a new proliferative marker in astrocytomas

Replication-dependent H3.1 and H3.2 histones are encoded by 11 genes. The H3 mRNA levels in brain astrocytomas using real-time RTPCR assay was examined. The sequence of primers and probe used in amplification was designed basing on the reference sequence GenBank accession no. Z83737. The H3 mRNA levels correlated with tumor grade (R=0.56, P-0.0012), Ki-67 proliferative antigen labeling index (R=0.58, P=0.0008) and patient survival time (R=-0.50, P=0.005), discriminating low-grade and high-grade tumors. Quantification of H3 mRNA with real-time RT-PCR using the proposed pair of primers may supplement classic proliferative tests and predictive factors in brain astrocytomas. (C) 2004 Elsevier B.V All rights reserved.