期刊
GENE
卷 333, 期 -, 页码 101-109出版社
ELSEVIER
DOI: 10.1016/j.gene.2004.02.015
关键词
flower development genes; orchidaceae; molecular evolutionary rates; molecular phylogeny
It has been suggested that the evolutionary analysis of floral development genes could explain the divergences between the rates of morphological and molecular evolution. The LEAFY (LFY) gene of Arabidopsis thaliana is one of the central regulatory genes in the control of flower development. We have identified the homologue of this gene (OrcLFY) in Orchis italica using 5'/3'RACE and primer walking, and compared the coding sequences of several orchid species. We analyzed nonsynonymous and synonymous substitution rates between the OrcLFYcoding regions of 14 species, and performed a McDonald-Kreitman test on Orchis morio and Orchis laxiflora populations, showing that purifying selection is acting on this gene in these orchids. We have performed a phylogenetic analysis showing that OrcLFY is a new useful marker to reconstruct molecular phylogenies at low taxonomic levels. (C) 2004 Elsevier B.V. All rights reserved.
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