期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1663, 期 1-2, 页码 97-107出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2004.02.004
关键词
flavone; chrysin; luteolin; myricetin; luteolin-7-glucoside; H-1 MAS NOESY; PFG NMR; lipid peroxidation
To investigate the structural basis for the antioxidative effects of plant flavonoids on the lipid molecules of cellular membranes, we have studied the location and distribution of five different flavonoid molecules (flavone, chrysin, luteolin, myricetin, and luteolin-7-glucoside) with varying polarity in monounsaturated model membranes. The investigated molecules differed in the number of hydroxyl groups attached to the polyphenolic benzo-gamma-pyrone compounds. To investigate the relation between hydrophobicity and membrane localization/orientation, we have applied H-1 magic angle spinning NMR techniques measuring ring current induced chemical shift changes, nuclear Overhauser enhancement cross-relaxation rates, and lateral diffusion coefficients. All investigated flavonoids show a broad distribution along the membrane normal with a maximum in the lipid/water interface. With increasing number of hydroxyl groups, the maximum of this distribution is biased towards the lipid headgroups. These results are confirmed by pulsed field gradient NMR measurements of the lateral diffusion coefficients of phospholipids and flavonoids, respectively. From the localization of different flavonoid protons in the membrane, a model for the orientation of the molecules in a lipid bilayer can be deduced. This orientation depends on the position of the polar center of the flavonoid molecule. (C) 2004 Elsevier B.V. All rights reserved.
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