Determination of some local anesthetics in human serum by gas chromatography with solid-phase extraction

A method of analysis based on solid-phase extraction coupled with capillary gas chromatographic system for determination of mepivacaine, bupivacaine and lidocaine from human serum was developed. As extraction sorbents were used Chromosorb 103, Tenax-GC and Chromosorb T. The best extraction sorbent proved to be Chromosorb 103. Their recoveries ranged from 91 to 94% at the target concentrations of approx. 1.5 mug ml(-1) in serum. Relative standard deviation of the recoveries ranged from 3.11 to 5.30 at these concentrations. As internal standard was used lidocaine. The chromatographic analysis was performed on a gas chromatograph equipped with a capillary column, HP-Innowax, and flame ionisation detector. Samples were injected in splitless mode. This method was applied in a stomatological clinic to healthy volunteers to whom superior-posterior alveolar nerve block anesthesia with mepivacaine was administered. (C) 2004 Elsevier B.V. All rights reserved.